CST, 79148S, Geminin (EM6) Mouse Monoclonal Antibody

Monoclonal Antibody for studying GMNN. Validated for IHC Leica Bond,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, Geminin (EM6) Mouse Monoclonal Antibody (CST #79148) is ready to ship. Product Usage Information IHC Leica Bond: 1:50 - 1:200 Immunohistochemistry (Paraffin): 1:50 Storage Supplied as liquid tissue culture supernatant containing sodium azide as a preservative. Stable for 6 months when stored at 4°C. Do not aliquot the antibody. Note: This product is only available in the format listed above. We are unable to provide this in a carrier free, custom formulation. Protocol Available protocols: IHC Leica Bond, Immunohistochemistry (Paraffin) Specificity / Sensitivity Geminin (EM6) Mouse Monoclonal Antibody recognizes endogenous levels of geminin protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a prokaryotic recombinant protein containing amino acids 5-209 of the mature protein. Background The initiation of DNA replication in mammalian cells is a highly coordinated process that ensures duplication of the genome only once per cell division cycle. Origins of replication are dispersed throughout the genome and their activities are regulated via the sequential binding of pre-replication and replication factors. The origin recognition complex (ORC) is thought to be bound to chromatin throughout the cell cycle (1,2). The pre-replication complex (pre-RC) forms in late mitosis/early G1 phase beginning with the binding of CDT1 and cdc6 to the origin, which allows binding of the heterohexameric MCM2-7 complex. Once this complex is formed, the origin is "licensed" for initiation of DNA replication. In order to ensure that replication occurs only once per cell cycle, geminin binds to and inhibits CDT1 during the S, G2, and M phases. This prevents the recruitment of the MCM complex to the origins of replication, which blocks the premature reformation of the pre-RC. At the metaphase/anaphase transition, geminin is degraded by the anaphase-promoting complex (APC) allowing for the formation of a new pre-RC (3,4). Alternate Names Gem; GEMI; Geminin; geminin DNA replication inhibitor; geminin, DNA replication inhibitor; GMNN; MGORS6; RP3-369A17.3 Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Mouse IgG2a