CST, 79154T, IKK gamma (F8I8M) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying IKK-gamma mouse. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, IKK gamma (F8I8M) Rabbit Monoclonal Antibody (CST #79154) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity IKK gamma (F8I8M) Rabbit Monoclonal Antibody recognizes endogenous levels of total IKKγ protein. A band of unknown origin is detected at 25 kDa in some cell lines. Species Reactivity: Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser178 of mouse IKKγ protein. Background The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins (1-3). Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB (3-7). The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits. IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regulatory subunit (8,9). Activation of IKK depends upon phosphorylation at Ser177 and Ser181 in the activation loop of IKKβ (Ser176 and Ser180 in IKKα), which causes conformational changes, resulting in kinase activation (10-13). Alternate Names 1110037D23Rik; AI848108; AI851264; AW124339; I-kappa-B kinase subunit gamma; IkB kinase subunit gamma; IkB kinase-associated protein 1; Ikbkg; IKK-gamma; IKK[g; IKK[g]; IKKAP1; IKKG; inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma; inhibitor of kappaB kinase gamma; Inhibitor of nuclear factor kappa-B kinase subunit gamma; mFIP-3; NEMO; NF-kappa-B essential modifier; NF-kappa-B essential modulator; OTTMUSP00000021358; OTTMUSP00000021360; OTTMUSP00000021361 Specification REACTIVITY: M R SENSITIVITY: Endogenous MW (kDa): 48-50 kDa Source/Isotype: Rabbit IgG