CST, 79210T, MDC1 (F6C8T) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MDC1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, MDC1 (F6C8T) Rabbit Monoclonal Antibody (CST #79210) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity MDC1 (F6C8T) Rabbit Monoclonal Antibody recognizes endogenous levels of total MDC1 protein. Based on the sequence of the peptide antigen, this antibody is expected to detect all isoforms of MDC1. This antibody detects an approximately 100 kDa protein of unknown identity in some cell lines. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro2076 of human MDC1 protein. Background Mediator of DNA damage checkpoint protein 1 (MDC1) is a scaffold protein that plays a central role in the early stages of the DNA damage response (DDR) and helps to maintain genomic stability (1). It is among the first proteins to accumulate at sites of DNA double-strand breaks (DSBs), where it forms a complex with phosphorylated histone H2AX (γ-H2AX), protecting its C-terminal tail from premature dephosphorylation until DSB repair is complete (1,2). Through phosphorylation-dependent interactions, MDC1 recruits DNA damage responsive E3 ubiquitin ligase RNF8, which ubiquitinates histones H2A and H2AX, leading to the accumulation of repair proteins, including 53BP1 and BRCA1, at the damage site (2-5). MDC1 also recruits E3 ubiquitin ligases Pellino-1 and -2 to DNA DSB sites and is essential for DNA damage-induced cell cycle arrest at the S and G2/M checkpoints (4-6). Due to its critical role in the DDR, MDC1 expression levels are being studied in relation to various cancers. Studies have shown that MDC1 may function as a tumor suppressor and MDC1 loss or reduction correlates with later-stage tumors, highlighting its potential as a biomarker for prognosis and therapeutic response (1,5). Alternate Names DKFZp781A0122; homologue to Drosophila photoreceptor protein calphotin; KIAA0170; MDC1; mediator of DNA damage checkpoint 1; Mediator of DNA damage checkpoint protein 1; mediator of DNA-damage checkpoint 1; MGC166888; NFBD1; Nuclear factor with BRCT domains 1 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 250-300 Source/Isotype: Rabbit IgG