CST, 79244S, SARS-CoV-2 ORF8 Antibody

Polyclonal Antibody for studying ORF8 protein SARS-CoV-2. Validated for Western Blotting. Highly specific and rigorously validated in-house, SARS-CoV-2 ORF8 Antibody (CST #79244) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity SARS-CoV-2 ORF8 Antibody recognizes transfected levels of total SARS-CoV-2 ORF8 protein. Species Reactivity: Virus Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro37 of SARS-CoV-2 ORF8 protein. Antibodies are purified by peptide affinity chromatography. Background The cause of the COVID-19 pandemic is a novel and highly pathogenic coronavirus, termed SARS-CoV-2 (severe acute respiratory syndrome coronavirus-2). SARS-CoV-2 is a member of the Coronaviridae family of viruses (1). The genome of SARS-CoV-2 is relatively large and encodes up to 29 open reading frames (ORFs). These include ORF1a and ORF1b (further processed into 16 non-structural proteins), 9 accessory proteins, and 4 canonical structural proteins: spike (S), envelope (E), membrane (M), and nucleocapsid (N) (2). ORF8 is transcribed and translated from an ORF in the nested subgenomic RNA of the SARS-CoV-2 genome. It is one of the most divergent proteins in the SARS-CoV genome, and is believed to interfere with the immune response by binding to MHC-1 molecules and targeting them for lysosomal degradation via autophagy (3,4). Alternate Names Non-structural protein 8; ns8; ORF8 protein; SARS-CoV-2 ORF8 protein Specification REACTIVITY: Vir SENSITIVITY: Transfected Only MW (kDa): 20 SOURCE: Rabbit