CST, 79253S, CD34 (ICO115) Mouse Monoclonal Antibody (PE Conjugate)

Monoclonal Antibody for studying CD34. Validated for Flow Cytometry (Live). Highly specific and rigorously validated in-house, CD34 (ICO115) Mouse Monoclonal Antibody (PE Conjugate) (CST #79253) is ready to ship. Product Usage Information Flow Cytometry (Live): 1:50 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Flow Cytometry (Live) Specificity / Sensitivity CD34 (ICO115) Mouse Monoclonal Antibody (PE Conjugate) detects endogenous levels of total CD34 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing BALB/c mice with blast cells of a chronic myeloid leukemia patient. Background CD34 is a type I transmembrane glycophosphoprotein expressed by hematopoietic stem/progenitor cells, vascular endothelium and some fibroblasts (1). CD34 expression has been the hallmark used to identify hematopoietic stem cells for many years. CD34+ hematopoietic stem cells expand and differentiate into all the lymphohematopoietic lineages upon cytokine or growth factor stimulation and lose CD34 expression upon differentiation. However, recent studies performed in various laboratories conflict with that convention (2). The extracellular domain of CD34 is homologous to CD43, a protein involved in cell-cell adhesion, and CD34 has been shown to function as a negative regulator of cell adhesion (3). CD34 associates with CrkL but not CrkII, is a substrate for PKC, and activation of PKC is coupled with surface expression of CD34 (1,4). Alternate Names CD34; CD34 antigen; CD34 molecule; Hematopoietic progenitor cell antigen CD34 Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Mouse IgG1