CST, 79299S, Angiotensinogen Antibody

Polyclonal Antibody for studying angiotensinogen. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, Angiotensinogen Antibody (CST #79299) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Angiotensinogen Antibody recognizes endogenous levels of total angiotensinogen protein. Species Reactivity: Human, Mouse Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of mouse angiotensinogen protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Angiotensinogen (AGT) is the primary precursor of angiotensins, peptide hormones that play a central role in the renin-angiotensin system (RAS) (1-3). AGT is a secreted protein synthesized primarily by the liver and secreted into circulation. Upon binding to renin, the amino terminal fragment of AGT is cleaved and released as a decapeptide hormone termed angiotensin 1 (Ang I). Ang I is subsequently processed by angiotensin converting enzyme (ACE) to generate angiotensin II (Ang II), which acts on AT1 and AT2 receptors in the central nervous system to increase production of anti-diuretic hormone (ADH), while promoting vasoconstriction in the peripheral circulation (4). Aberrant upregulation of Ang II has been associated with numerous clinical conditions, including hypertension, atherosclerosis, myocardial hypertrophy, and obesity (5-7). Alternative cleavage products of Ang I (e.g., Ang 1-7) can also be generated by ACE2 cleavage, some of which display biological functions that are distinct from Ang II (8). Treatments that target the RAS (e.g., ACE inhibitors) are consequently of significant importance in the treatment of hypertensive and hypertensive-related disorders(5-8). Alternate Names AGT; alpha-1 antiproteinase, antitrypsin; Ang I; Ang II; Ang III; Ang IV; angiotensin; Angiotensin 1-10; Angiotensin 1-4; Angiotensin 1-5; Angiotensin 1-7; Angiotensin 1-8; Angiotensin 1-9; Angiotensin 2-8; Angiotensin 3-8; Angiotensin I; Angiotensin II; Angiotensin III; Angiotensin IV; Angiotensin-1; Angiotensin-2; Angiotensin-3; Angiotensin-4; Angiotensinogen; angiotensinogen (serpin peptidase inhibitor, clade A, member 8); ANGT; ANHU; Des-Asp[1]-angiotensin II; fetal-liver predominant transporter 1; FLJ92595; FLJ97926; hFLT1; pre-angiotensinogen; serine (or cysteine) proteinase inhibitor; Serpin A8; serpin peptidase inhibitor, clade A, member 8; SERPINA8 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 55, 70 SOURCE: Rabbit