CST, 79462T, U2AF2 (E6M3G) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying U2AF2. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, U2AF2 (E6M3G) Rabbit Monoclonal Antibody (CST #79462) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity U2AF2 (E6M3G) Rabbit Monoclonal Antibody recognizes endogenous levels of total U2AF2 protein. This antibody recognizes U2AF2 protein cleavage products around 43 and 46 kDa. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val275 of human U2AF2 protein. Background U2 small nuclear RNA auxiliary factor 2 (U2AF2) is the large (65 kDa) subunit of the U2 auxiliary factor (U2AF) that plays an essential role in the splicing of pre-mRNA to generate functional mRNA transcripts. U2AF2 forms a heterodimer with the small (35 kDa) U2AF1 subunit to create the U2AF that recognizes the 3' splice site and facilitates spliceosome assembly (1-3). Research studies indicate that U2AF1 binds to the 3' splice site consensus AG dinucleotide at the intron-exon boundary while U2AF2 recognizes and binds the polypyrimidine tract upstream of the 3' splice site. These two steps ensure accurate spliceosome assembly at splice sites (4-6). Mutations in , which alter its splicing capabilities, have been described in various cancers (7,8). Alternate Names hU2AF(65); hU2AF65; splicing factor U2AF 65 kD subunit; Splicing factor U2AF 65 kDa subunit; U2 (RNU2) small nuclear RNA auxiliary factor 2; U2 auxiliary factor 65 kDa subunit; U2 small nuclear ribonucleoprotein auxiliary factor (65kD); U2 small nuclear RNA auxiliary factor 2; U2 snRNP auxiliary factor large subunit; U2AF2; U2AF65 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 43, 46, 65 Source/Isotype: Rabbit IgG