CST, 79479S, MYH11 (E4Q4L) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MYH11. Validated for Western Blotting,Immunofluorescence (Frozen),Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, MYH11 (E4Q4L) Rabbit Monoclonal Antibody (CST #79479) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Frozen): 1:400 - 1:1600 Immunofluorescence (Immunocytochemistry): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 40399 . Protocol Available protocols: Western Blotting, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity MYH11 (E4Q4L) Rabbit Monoclonal Antibody recognizes endogenous levels of total MYH11 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu639 of human MYH11 protein. Background encodes the smooth muscle myosin heavy chain (SMMHC), a major specific contractile protein produced in smooth muscle (1). Mutations in cause medial degeneration of the aorta with very low smooth muscle cell (SMC) content. Disease symptoms include aortic stiffness, aneurysm/dissection, and ductus arteriosus (2). One of the most frequent chromosomal translocations associated with acute myeloid leukemia (AML) is the fusion of , encoding the core binding protein CBFβ transcriptional factor, to (3). This gene fusion event results in a chimeric protein that suppresses the transactivation mediated by CBF/AML-1, impairs the process of normal hematopoietic differentiation (4,5), and creates preleukemic myeloid progenitors that can be targets for AML transformation (6). Alternate Names AAT4; DKFZp686D10126; DKFZp686D19237; epididymis secretory sperm binding protein; FAA4; FLJ35232; KIAA0866; MGC126726; MGC32963; MYH11; Myosin heavy chain 11; Myosin heavy chain, smooth muscle isoform; Myosin-11; myosin, heavy chain 11, smooth muscle; myosin, heavy polypeptide 11, smooth muscle; SMHC; SMMHC Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 270 Source/Isotype: Rabbit IgG