CST, 79485T, TRBC1/TCR beta constant region 1 (E6Z3S) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TCRbeta. Validated for Western Blotting,IHC Leica Bond,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, TRBC1/TCR beta constant region 1 (E6Z3S) Rabbit Monoclonal Antibody (CST #79485) is ready to ship. Product Usage Information Western Blotting: 1:1000 IHC Leica Bond: 1:400 - 1:1600 Immunohistochemistry (Paraffin): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 15503 . Protocol Available protocols: Western Blotting, IHC Leica Bond, Immunohistochemistry (Paraffin) Specificity / Sensitivity TRBC1/TCR beta constant region 1 (E6Z3S) Rabbit Monoclonal Antibody recognizes endogenous levels of total TRBC1/TCRβ constant region 1 protein. This antibody does not cross-react with TRBC2/TCRβ constant region 2 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human TRBC1/TCRβ constant region 1 protein. Background The αβ T Cell Receptor (TCR) is a heterodimer composed of an alpha chain and a beta chain expressed on the surface of T cells (1,2). It detects peptide antigen presented by major histocompatibility complex (MHC) molecules (1,2). Detection of cognate antigen leads to activation of the TCR signaling pathway through the CD3 signaling chains that associate with the TCR (1). The TCR chains are made up of a constant region and variable regions that get rearranged during T cell development in the thymus (3). There are two TCRβ constant genes, and (3). A population of normal T cells is polyclonal and contains a mix of TRBC1- and TRBC2-expressing T cells, while T cell malignancies are monoclonal and all express the same TCR which either contains TRBC1 or TRBC2 (4). Therefore, it is possible that T cell malignancies could be identified and targeted with reagents or therapies that can distinguish between TRBC1 and TRBC2 (4). Alternate Names BV05S1J2.2; MGC88817; T cell receptor beta constant 1; T-cell receptor beta-1 chain C region; TCR-beta1; TCRB; TCRBC1; TCRbeta; TCRbeta1; TRBC1; V_segment translation product Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 30-40 Source/Isotype: Rabbit IgG