CST, 79501S, CD79A (F3Z1H) Rabbit Monoclonal Antibody (Alexa Fluor® 488 Conjugate)

Monoclonal Antibody for studying CD79A mouse. Validated for Immunohistochemistry (Paraffin),Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, CD79A (F3Z1H) Rabbit Monoclonal Antibody (Alexa Fluor^® 488 Conjugate) (CST #79501) is ready to ship. Product Usage Information Immunohistochemistry (Paraffin): 1:50 - 1:200 Flow Cytometry (Fixed/Permeabilized): 1:200 - 1:800 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Immunohistochemistry (Paraffin), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity CD79A (F3Z1H) Rabbit Monoclonal Antibody (Alexa Fluor Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of mouse CD79A protein. Background Antigen receptors found on the surface of B cells contain a heterodimeric signaling component composed of CD79A and CD79B, also known as Ig α and Ig β, respectively (1,2). Presence of this receptor complex is essential for B cell development and function (3). Together these two proteins and the associated B cell receptor (BCR) initiate intracellular signaling following antigen binding (4,5). An immunoreceptor tyrosine-based activation motif (ITAM) found in the CD79A intracellular region appears to be important for its function (6). Antigen binding precedes formation of the CD79A and CD79B heterodimer and subsequent activation of receptor associated kinases (7). Research has shown that CD79A is a marker for B-lineage lymphoblastic leukemia (8). Additionally, investigators have found that mutations in the ( ) gene are associated with abnormally low levels of functional B cell receptors in some cases of chronic B cell lymphocytic leukemia (9). Alternate Names B-cell antigen receptor complex-associated protein alpha chain; Cd79; Cd79a; CD79A antigen (immunoglobulin-associated alpha); Ig alpha; Ig-; Ig-alpha; Iga; Igalpha; immunoglobulin-associated alpha; Ly-5; Ly-54; Ly5; Ly54; Mb-1; MB-1 membrane glycoprotein; Membrane-bound immunoglobulin-associated protein; Surface IgM-associated protein Specification REACTIVITY: M SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG