CST, 79519S, Neurogranin (E9L8D) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying neurogranin. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Frozen). Available in 2 sizes. Highly specific and rigorously validated in-house, Neurogranin (E9L8D) Rabbit Monoclonal Antibody (CST #79519) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Immunofluorescence (Frozen): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 85207 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Frozen) Specificity / Sensitivity Neurogranin (E9L8D) Rabbit Monoclonal Antibody recognizes endogenous levels of total neurogranin protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu17 of human neurogranin protein. Background Neurogranin is a small neuronal protein that binds to calmodulin in the absence of Ca (1,2). Calmodulin binds Ca and acts as a Ca sensor to drive Ca -dependent signal transduction pathways by modulating its interaction with various kinases and phosphatases (3). In the postsynaptic compartment of the neuron, Ca  is carefully regulated and is a critical regulator of synaptic function and plasticity (4). Neurogranin is primarily expressed in the brain and is enriched in somato-dendritic compartments of projection neurons in various regions of the brain (5). Postsynaptically-enriched neurogranin likely influences Ca  or Ca /calmodulin-dependent neuronal functions, including synaptic plasticity, by binding to and releasing calmodulin in a Ca -dependent manner (6-9). Genetic variants in the gene encoding neurogranin, are linked to several neuropsychiatric diseases, including schizophrenia (10). Interestingly, increase in cerebrospinal fluid neurogranin is correlated with neurodegenerative disease progression, which suggests that neurogranin could act as a biomarker for diseases like Alzheimer's disease (11). Alternate Names calmodulin-binding protein; hng; NEUG; NEUG(55-78); Neurogranin; neurogranin (protein kinase C substrate, RC3); Ng; NRGN; protein kinase C substrate; RC3 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 15 Source/Isotype: Rabbit IgG