CST, 79606S, PCGF3 (F3X9C) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PCGF3. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, PCGF3 (F3X9C) Rabbit Monoclonal Antibody (CST #79606) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity PCGF3 (F3X9C) Rabbit Monoclonal Antibody recognizes endogenous levels of total PCGF3 protein. This antibody does not cross-react with PCGF5 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro64 of human PCGF3 protein. Background Polycomb group RING finger homologs (PCGF1-6) are essential proteins for the assembly of the polycomb repressive complex 1 (PRC1) group of complexes. RING1A/B and a unique PCGF protein make up the catalytic core of PRC1 to ubiquitinate histone H2A. Differences in binding specificities give PRC1 complexes unique functions depending on which PCGF is included (1-3). PCGF3 is closely related to PCGF5 and recruits PRC1 and PRC2 in response to Xist expression (4). PCGF3/5 can act as transcriptional activators in embryonic stem cells, where they interact with Tex10, a pluripotency and super-enhancer regulator (5). Alternate Names DKFZp686D20235; DONG1; FLJ36550; FLJ43813; MGC129615; MGC40413; PCGF3; polycomb group ring finger 3; Polycomb group RING finger protein 3; ring finger protein 3; RING finger protein 3A; RNF3; RNF3A Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 28 Source/Isotype: Rabbit IgG