CST, 7963S, PathScan® Phospho-Src (Tyr416) Sandwich ELISA Antibody Pair

ELISA Antibody Pair for studying Src (Tyr419) phosphate in the research area. Storage Capture and detection antibodies are stored at 4°C. HRP-linked secondary reagent is stored at -20°C. Protocol Available protocols: ELISA+ Specificity / Sensitivity For Antibody Pair specificity and sensitivity, please refer to the corresponding PathScan Species Reactivity: Human, Dog Background The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk, and Hck, are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr530 in the carboxy-terminal tail by Csk renders the enzyme less active (2). Alternate Names cell based assay; cell-based assay; protein tyrosine kinase; receptor tyrosine kinase; sandwich ELISA; screening; Src Specification REACTIVITY: H Dg