CST, 79762S, LXR-alpha (E3D9B) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying LXR-alpha. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, LXR-alpha (E3D9B) Rabbit Monoclonal Antibody (CST #79762) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity LXR-alpha (E3D9B) Rabbit Monoclonal Antibody recognizes overexpressed levels of total LXR-α protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human LXR-α protein. Background Liver X receptors LXR-α and LXR-β are nuclear hormone receptor superfamily members responsible for regulating expression of target genes that control cholesterol transport and metabolism (1). When bound by the oxidized derivatives of cholesterol (oxysterols), activated LXR receptors function as sterol sensors to regulate transcription of the genes involved in the cholesterol homeostasis (1,2). The LXR-α protein is expressed at high levels in rat liver, kidney, intestine, adipose, and spleen; LXR-β is more ubiquitously expressed within rat tissues (1,3). Research studies indicate that glucose binds and upregulates the transcriptional activity of LXR-α and LXR-β (4). LXR-α and LXR-β are putative glucose sensors that integrate glucose metabolism and fatty acid biosynthesis in the liver (4). Additional studies show that female mice deficient in LXR-β develop gallbladder cancer (5). In addition, LXR-β plays a role in protecting dopaminergic neurons in a Parkinson's disease model (6). Alternate Names liver X nuclear receptor alpha variant 1; Liver X receptor alpha; liver X receptor-alpha; liver X receptor, alpha; LXR-a; LXR-alpha; LXRA; NR1H3; Nuclear receptor subfamily 1 group H member 3; nuclear receptor subfamily 1, group H, member 3; Oxysterols receptor LXR-alpha; RLD-1 Specification REACTIVITY: H SENSITIVITY: Transfected Only MW (kDa): 48,52 Source/Isotype: Rabbit IgG