CST, 79764S, BCAT2 (D8K3O) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying BCAT2. Validated for WB,IP,IHC,eCLIP. Available in 2 sizes. Highly specific and rigorously validated in-house, BCAT2 (D8K3O) Rabbit Monoclonal Antibody (CST #79764) is ready to ship. Product Usage Information For more information about the RBP-eCLIP service please visit Eclipsebio . Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunohistochemistry (Paraffin): 1:800 eCLIP: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin) Specificity / Sensitivity BCAT2 (D8K3O) Rabbit Monoclonal Antibody recognizes endogenous levels of total BCAT2 protein. This antibody does not cross-react with BCAT1 protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu39 of human BCAT2 protein. Background BCAT1 and BCAT2 are cytosolic and mitochondrial branched chain aminotransferases, respectively (1,2). Research studies have implicated BCAT1 in distant metastasis in patients with advanced colorectal cancer (3). Disruption of BCAT2 in mice leads to higher levels of plasma branched-chain amino acids, reduced adiposity and body weight, and increased energy expenditure, suggesting its role in regulating insulin sensitivity (4). Alternate Names BCAM; BCAT(m); BCAT2; BCATM; BCT2; branched chain amino acid transaminase 2; branched chain amino-acid transaminase 2, mitochondrial; branched chain aminotransferase 2, mitochondrial; branched-chain amino acid aminotransferase; Branched-chain-amino-acid aminotransferase, mitochondrial; ECA40; HVLI; Placental protein 18; PP18 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 39 Source/Isotype: Rabbit IgG