CST, 79769T, HLA-G (E8N9C) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying HLA-G. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, HLA-G (E8N9C) Rabbit Monoclonal Antibody (CST #79769) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 49508 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity HLA-G (E8N9C) Rabbit Monoclonal Antibody recognizes endogenous levels of total HLA-G protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu102 of human HLA-G protein. Background HLA-G (HLA-G histocompatibility antigen, class I, G) is a non-classical MHC molecule expressed by trophoblasts in placenta, thymic epithelial cells, and some tumors. Alternative splicing leads to generation of at least six isoforms, four that are transmembrane proteins and two that are soluble (1-4). It is an inhibitory molecule involved in immune tolerance and escape, originally studied for its role in maternal tolerance of the fetus during pregnancy (1-5). HLA-G binds ILT2, ILT4, and KIR2DL4, playing a role in the regulation of natural killer, T, and monocyte/macrophage cells (4-5). Its involvement in evasion of immune response makes it a potential target for immunotherapy (6). Alternate Names b2 microglobulin; HLA class I histocompatibility antigen, alpha chain G; HLA class I histocompatibility antigen, alpha chain G precursor; HLA G antigen; HLA-6.0; HLA-G; HLA-G histocompatibility antigen, class I, G; HLAG; major histocompatibility complex, class I, G; MHC class I antigen G; MHC class Ib antigen; MHC-G; mutant MHC class I antigen; mutant MHC class Ib antigen; sHLA-G; Soluble HLA class I histocompatibility antigen, alpha chain G Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 30-40 Source/Isotype: Rabbit IgG