CST, 79799S, SETD6 (F5G6P) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SETD6. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, SETD6 (F5G6P) Rabbit Monoclonal Antibody (CST #79799) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity SETD6 (F5G6P) Rabbit Monoclonal Antibody recognizes endogenous levels of total SETD6 protein. This antibody detects an 85 kDa protein of unknown identity in some cell lines. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human SETD6 protein. Background Lysine methylation is a dynamic posttranslational modification that regulates protein function and various biological processes. SET domain-containing protein 6 (SETD6) is a mono-methyltransferase that promotes the methylation of multiple proteins involved in the regulation of gene expression programs (1). Unlike other lysine methyltransferases (KMTs), SETD6 was first discovered as a non-histone KMT and has been shown to methylate key regulatory factors such as RELA, TWIST1, and BRD4 (2-4). SETD6 can exist in monomer, oligomer, and higher-molecular-weight aggregate forms and its auto-methylation activity is key in stabilizing oligomerization and enzymatic activity (5). Moreover, SETD6 expression is misregulated in a number of cancers. Namely, SETD6 protein expression is upregulated in lung adenocarcinoma tumors, several bladder cancer cell lines, oral squamous cell carcinoma, and in samples from glioma patients (3,6-8). Alternate Names FLJ21148; N-lysine methyltransferase SETD6; SET domain containing 6; SET domain containing 6, protein lysine methyltransferase; SET domain-containing protein 6; SETD6 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 55 Source/Isotype: Rabbit IgG