CST, 79800SF, Phospho-VEGF Receptor 2 (Tyr1175) (19A10) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying VEGFR2 (Tyr1175) phosphate. Validated for Western Blotting,ELISA+. Highly specific and rigorously validated in-house, Phospho-VEGF Receptor 2 (Tyr1175) (19A10) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #79800) is ready to ship. Product Usage Information This product is the carrier free version of product #2478. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 2478 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Phospho-VEGF Receptor 2 (Tyr1175) (19A10) Rabbit Monoclonal Antibody (BSA and Azide Free) detects endogenous levels of VEGFR-2 proteins only when phosphorylated at tyrosine 1175. This antibody may cross-react with VEGFR1. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr1175 of human VEGF receptor-2. Background Vascular endothelial growth factor receptor 2 (VEGFR2, KDR, Flk-1) is a major receptor for VEGF-induced signaling in endothelial cells. Upon ligand binding, VEGFR2 undergoes autophosphorylation and becomes activated (1). Major autophosphorylation sites of VEGFR2 are located in the kinase insert domain (Tyr951/996) and in the tyrosine kinase catalytic domain (Tyr1054/1059) (2). Activation of the receptor leads to rapid recruitment of adaptor proteins, including Shc, GRB2, PI3 kinase, NCK, and the protein tyrosine phosphatases SHP-1 and SHP-2 (3). Phosphorylation at Tyr1212 provides a docking site for GRB2 binding and phospho-Tyr1175 binds the p85 subunit of PI3 kinase and PLCγ, as well as Shb (1,4,5). Signaling from VEGFR2 is necessary for the execution of VEGF-stimulated proliferation, chemotaxis and sprouting, as well as survival of cultured endothelial cells and angiogenesis (6-8). Alternate Names CD309; Fetal liver kinase 1; fetal liver kinase-1; FLK-1; FLK1; KDR; Kinase insert domain receptor; kinase insert domain receptor (a type III receptor tyrosine kinase); Protein-tyrosine kinase receptor flk-1; soluble VEGFR2; tyrosine kinase growth factor receptor; Vascular endothelial growth factor receptor 2; VEGFR; VEGFR-2; VEGFR2; VGFR2 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 230 Source/Isotype: Rabbit IgG