CST, 7980V2, PathScan® Phospho-eNOS (Ser1177) Sandwich ELISA Kit

ELISA Kit for studying eNOS (Ser1177) phosphate in the research area. Protocol Available protocols: ELISA+ Specificity / Sensitivity PathScan Species Reactivity: Bovine Background Endothelial nitric-oxide synthase (eNOS) is an important enzyme in the cardiovascular system. It catalyzes the production of nitric oxide (NO), a key regulator of blood pressure, vascular remodeling, and angiogenesis (1,2). The activity of eNOS is regulated by phosphorylation at multiple sites. The two most thoroughly studied sites are the activation site Ser1177 and the inhibitory site Thr495 (3). Several protein kinases including Akt/PKB, PKA, and AMPK activate eNOS by phosphorylating Ser1177 in response to various stimuli (4,5). In contrast, bradykinin and H O activate eNOS activity by promoting both Ser1177 phosphorylation and Thr495 dephosphorylation (6,7). Alternate Names cNOS; Constitutive NOS; EC-NOS; ECNOS; Endothelial NOS; eNOS; Nitric oxide synthase 3; nitric oxide synthase 3 (endothelial cell); nitric oxide synthase 3 transcript variant eNOS-delta20; nitric oxide synthase 3 transcript variant eNOS-delta20-21; nitric oxide synthase 3 transcript variant eNOS-delta21; Nitric oxide synthase, endothelial; NOS type III; NOS3; NOSIII Specification REACTIVITY: B