CST, 79842S, ZDHHC5 (E7T4N) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ZDHHC5. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, ZDHHC5 (E7T4N) Rabbit Monoclonal Antibody (CST #79842) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity ZDHHC5 (E7T4N) Rabbit Monoclonal Antibody recognizes endogenous levels of total ZDHHC5 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu330 of human ZDHHC5 protein. Background Zinc finger DHHC domain-containing protein 5 (ZDHHC5) is a multipass membrane protein that is localized predominantly at the plasma membrane and endosomal compartment membranes (1). ZDHHC5 belongs to a large family of enzymes known as palmitoyl-acyltransferases. Using long-chain fatty acids such as palmitic acid, ZDHHC5 catalyzes the reversible, post-translational modification of cysteine residues within protein substrates to regulate their trafficking and subcellular localization (1,2). Research studies have shown that ZDHHC5 is itself regulated by multiple types of post-translational modifications, such as phosphorylation and palmitoylation, which regulate its subcellular localization and enzymatic activity (3,4). Palmitoylation mediated by ZDHHC5 plays a role in the host response to pathogens. Indeed, ZDHHC5-mediated palmitoylation of NOD1/2 pattern recognition receptors facilitates their correct localization to endosomal vesicle membranes containing internalized bacteria in order to initiate innate immune signaling cascades (5). Furthermore, ZDHHC5 was recently shown to interact with SARS-CoV-2 spike protein. This interaction is posited to facilitate S-acylation of the spike protein and fusion with host cell membranes (6,7). Alternate Names DHHC-5; DHHC5; DKFZp586K0524; KIAA1748; membrane-associated DHHC5 zinc finger protein; PALMITOYLTRANSFERASE; Palmitoyltransferase ZDHHC5; Probable palmitoyltransferase ZDHHC5; ZDHC5; ZDHHC5; Zinc finger DHHC domain-containing protein 5; zinc finger DHHC-type containing 5; zinc finger DHHC-type palmitoyltransferase 5; Zinc finger protein 375; zinc finger, DHHC domain containing 5; zinc finger, DHHC-type containing 5; ZNF375 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 78 Source/Isotype: Rabbit IgG