CST, 79846SF, GPNMB (E4D7P) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying GPNMB. Validated for Western Blotting,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, GPNMB (E4D7P) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #79846) is ready to ship. Product Usage Information This product is the carrier free version of product #38313. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 38313 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity GPNMB (E4D7P) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total GPNMB protein. Based upon sequence alignment, this antibody is not predicted to cross-react with PMEL. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human GPNMB protein. Background Glycoprotein non-metastatic gene B (GPNMB) is a type I transmembrane glycoprotein overexpressed in many types of cancer. The GPNMB glycoprotein is involved in many physiological processes, including mediating transport of late melanosomes to keratinocytes (1), regulating osteoblast and osteoclast differentiation and function (2), stimulating dendritic cell maturation, promoting adhesion of dendritic cells to endothelial cells (3), enhancing autophagosome fusion to lysosomes in tissue repair, and regulating degradation of cellular debris (4,5). While typical GPNMB expression is seen in tissues including skin, heart, kidney, lung, liver, and skeletal muscle (3,6), research studies show elevated GPNMB expression often contributes to the metastatic phenotype in numerous cancers (reviewed in 7). GPNMB is typically localized to intracellular compartments in normal cells (1,8), but investigators found it primarily on the cell surface of tumor cells (9,10). Differential localization and expression, and the role of GPNMB as a tumor promoter in many cancer types make this protein a viable therapeutic target (11). The GPNMB ectodomain can be cleaved by matrix metalloproteinases and shed from the cell surface (12). Research studies identify the sheddase ADAM10 as one peptidase responsible for cleavage of the GPNMB ectodomain at the surface of breast cancer cells. Shedded GPNMB ectodomains may promote angiogenesis by inducing endothelial cell migration (13). Alternate Names glycoprotein (transmembrane) nmb; glycoprotein NMB; glycoprotein nmb-like protein; glycoprotein nonmetastatic melanoma protein B; GPNMB; Hematopoietic growth factor inducible neurokinin-1 type; HGFIN; NMB; osteoactivin; PLCA3; Transmembrane glycoprotein HGFIN; Transmembrane glycoprotein NMB Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 95, 120 Source/Isotype: Rabbit IgG