CST, 79877S, SHIP1 (E8M5D) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SHIP1 mouse. Validated for Western Blotting,Immunofluorescence (Frozen),Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, SHIP1 (E8M5D) Rabbit Monoclonal Antibody (CST #79877) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Frozen): 1:100 - 1:200 Immunofluorescence (Immunocytochemistry): 1:800 - 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity SHIP1 (E8M5D) Rabbit Monoclonal Antibody recognizes endogenous levels of total SHIP1 protein. This antibody does not cross-react with human SHIP1 protein. A band of unknown identity is observed at 70 kDa by western blot. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val1067 of mouse SHIP1 protein. Background SH2-containing inositol phosphatase 1 (SHIP1) is a hematopoietic phosphatase that hydrolyzes phosphatidylinositol-3,4,5-triphosphate to phosphatidylinositol-3,4-bisphosphate (1). SHIP1 is a cytosolic phosphatase with an SH2 domain in its amino terminus and two NPXY Shc binding motifs in its carboxy terminus (1,2). Upon receptor cross-linking, SHIP is first recruited to the membrane junction through binding of its SH2 domain to the phospho-tyrosine in the ITIM motif (2), followed by tyrosine phosphorylation on the NPXY motif (2). The membrane relocalization and phosphorylation on the NPXY motif is essential for the regulatory function of SHIP1 (3-5). Its effect on calcium flux, cell survival, growth, cell cycle arrest, and apoptosis is mediated through the PI3K and Akt pathways (3-5). Tyr1022 is located in one of the NPXY motifs in SHIP1, and its phosphorylation is important for SHIP1 function (6). Alternate Names 7a33; AI323613; inositol polyphosphate-5-phosphatase D; Inositol polyphosphate-5-phosphatase of 145 kDa; inositol polyphosphate-5-phosphatase, 145 kDa; Inpp5d; MGC117680; p150Ship; Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1; Phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase 1; Phosphatidylinositol-4,5-bisphosphate 5-phosphatase; s-SHIP; SH2 containing inositol phosphotase; SH2 domain-containing inositol 5'-phosphatase 1; SH2 domain-containing inositol phosphatase 1; SH2 domain-containing inositol-5'-phosphatase 1; SH2-containing inositol phosphatase SHIP; SHI; Ship; SHIP-1; Ship1; SIP-145; Src homology 2 domain-containing inositol-5-phosphatase Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 145 Source/Isotype: Rabbit IgG