CST, 79925SF, Brachyury (D2Z3J) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying Brachyury. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, Brachyury (D2Z3J) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #79925) is ready to ship. Product Usage Information This product is the carrier free version of product #81694. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 81694 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Brachyury (D2Z3J) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total Brachyury protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro256 of human Brachyury protein. Background Brachyury protein, encoded by the gene, is a transcription factor that is vital for the formation of posterior mesoderm and axial development during vertebrate embryogenesis (1). In the mouse, brachyury is necessary for mesodermal morphogenetic cell movements during gastrulation. Brachyury mutant mice die and display deficient mesoderm formation including an abnormal notochord, missing posterior somites, and a reduced allantois (2). Human brachyury is expressed in the notochord, as well as in chordoma tumors that occur along the spine, making it a good marker for notochord and notochord-derived tumors (3,4). A common polymorphism in the human gene has also been shown to be associated with development of the multifactorial neural tube defect, spina bifida (5,6). Alternate Names BRAC; Brachyury; Brachyury protein; MGC104817; OTTHUMP00000017588; Protein T; SAVA; T; T brachyury; T brachyury transcription factor; T brachyury-like; T-box transcription factor T; T, brachyury homolog; T, brachyury homolog (mouse); TBXT; TFT; transcription factor T Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 54 Source/Isotype: Rabbit IgG