CST, 79979SF, Perilipin-2 (E6G6M) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying perilipin 2. Validated for Western Blotting,Immunohistochemistry (Paraffin),Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, Perilipin-2 (E6G6M) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #79979) is ready to ship. Product Usage Information This product is the carrier free version of product #95109. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 95109 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Perilipin-2 (E6G6M) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total perilipin-2 protein. This antibody does not cross-react with perilipin-1 protein. Non-specific staining was observed in brain by immunohistochemistry. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human perilipin-2 protein. Background Eukaryotic cells store lipids in lipid droplets. These intracellular lipid storage structures are covered by members of the perilipin family of lipid droplet coat proteins. Perilipins establish an interface between stored lipids and the cytosol, and coordinate lipid storage and utilization (1-3). Mice deficient in perilipin-2 have decreased levels of triglycerides in the liver and are protected from fatty liver disease. In addition, these mice show increased autophagy in the liver. In contrast, overexpression of perilipin-2 protects lipid droplets from autophagy (4). Alternate Names ADFP; Adipophilin; Adipose differentiation-related protein; ADRP; MGC10598; perilipin 2; Perilipin-2; PLIN2 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 52 Source/Isotype: Rabbit IgG