CST, 7998S, HELLS Antibody

Polyclonal Antibody for studying LSH. Validated for Western Blotting. Highly specific and rigorously validated in-house, HELLS Antibody (CST #7998) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity HELLS Antibody recognizes endogenous levels of total HELLS protein. This antibody is predicted to detect multiple isoforms of this protein. Species Reactivity: Human, Mouse, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys520 of human HELLS protein. Antibodies are purified by protein A and peptide affinity chromatography. Background HELLS, or LSH1, is a lymphoid-specific helicase thought to be involved in cellular proliferation and leukemogenesis (1,2). It is believed to be a chromatin remodeler and is required for DNMT1-mediated methylation maintenance and DNMT3A/DNMT3B-mediated methylation. The role of HELLS in methylation maintenance was thought to be largely confined to repetitive DNA sequences, including major and minor satellite sequences, rather than single copy genes (3,4); recent evidence suggests a role in genome-wide cysteine methylation at non-repeat sequences (5). methylation maintenance is associated with silencing of specific genes, some known to be involved in pluripotency and lineage commitment (6,7). Alternate Names FLJ10339; helicase, lymphoid specific; helicase, lymphoid-specific; HELLS; ICF4; LSH; Lymphoid-specific helicase; Nbla10143; PASG; Proliferation-associated SNF2-like protein; SMARCA6; SWI/SNF2-related matrix-associated actin-dependent regulator of chromatin subfamily A member 6; SWI/SNF2-related, matrix-associated, actin-dependent regulator of chromatin, subfamily A, member 6 Specification REACTIVITY: H M Mk SENSITIVITY: Endogenous MW (kDa): 97 SOURCE: Rabbit