CST, 79990S, ROD1 (E6B2J) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ROD1. Validated for Western Blotting,Simple Western™. Available in 2 sizes. Highly specific and rigorously validated in-house, ROD1 (E6B2J) Rabbit Monoclonal Antibody (CST #79990) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity ROD1 (E6B2J) Rabbit Monoclonal Antibody recognizes endogenous levels of total ROD1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly422 of human ROD1 protein. Background ROD1, also known as PTBP3, is a member of the polypyrimidine tract-binding (PTB) protein family and a critical RNA-binding factor (1). Characterization of the mRNA and protein isoforms of ROD1 has revealed that alternative splicing surrounding the 5′ end of the gene leads to the generation of eight mRNA and three major protein isoforms. Specifically, alternative translation by re-initiation and leaky scanning through downstream start codons leads to the generation of up to three protein isoforms with varying domains and RNA-binding properties (2). ROD1 has been shown to play functional roles in RNA splicing, translational activation, and mRNA stability and is deregulated in numerous cancers, including breast, gastric, and colorectal cancer (3-6). Alternate Names DKFZp781I1117; fission yeast differentiation regulator; polypyrimidine tract binding protein 3; Polypyrimidine tract-binding protein 3; PTBP3; Regulator of differentiation 1; ROD1; ROD1 regulator of differentiation 1; ROD1 regulator of differentiation 1 (S. pombe) Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 48, 58 Source/Isotype: Rabbit IgG