CST, 80063SF, Glutamate Dehydrogenase 1/2 (D9F7P) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying GLUD1. Validated for Western Blotting,Immunohistochemistry (Paraffin),Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, Glutamate Dehydrogenase 1/2 (D9F7P) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #80063) is ready to ship. Product Usage Information This product is the carrier free version of product #12793. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 12793 . Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Glutamate Dehydrogenase 1/2 (D9F7P) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total glutamate dehydrogenase 1 and 2 proteins. Species cross-reactivity for IHC-P and IF-IC is in human only. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro190 of human glutamate dehydrogenase 1 protein. Background Glutamate dehydrogenase is a mitochondrial enzyme that catalyzes the oxidative deamination of glutamate to α-ketoglutarate through association with the cofactor nicotinamide adenine dinucleotide phosphate (1). Glutamate dehydrogenase is highly expressed in various tissues such as the liver, brain, kidney, heart, pancreas, ovaries, and testis. Two isoforms produced by two distinct genes are found in mammalian tissues. The gene is ubiquitously expressed (2), while the gene is specifically expressed in testicular tissues and astrocytes (3,4). Glutamate dehydrogenase links glutamate to the Krebs cycle, thereby playing a critical role in the regulation of energy homeostasis. Research studies have shown that changes in glutamate dehydrogenase activity in pancreatic β-cells can cause a hyperinsulinism syndrome (5). Alternate Names DHE3; epididymis secretory sperm binding protein; epididymis tissue sperm binding protein Li 18mP; GDH; GDH 1; GDH1; GLUD; GLUD1; glutamate dehydrogenase (NAD(P)+); glutamate dehydrogenase 1; Glutamate dehydrogenase 1, mitochondrial; MGC132003 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 52 Source/Isotype: Rabbit IgG