CST, 80075T, SP100 (F1H6X) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SP100. Validated for WB,WB,IP,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, SP100 (F1H6X) Rabbit Monoclonal Antibody (CST #80075) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:100 Immunofluorescence (Immunocytochemistry): 1:6400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity SP100 (F1H6X) Rabbit Monoclonal Antibody recognizes endogenous levels of total SP100 protein. This antibody recognizes 7 isoforms of SP100, including SP100-HMG, SP100-A, SP100-B, and SP100-C. This antibody detects a 20 kDa protein of unknown identity in some cell lines. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro382 of human SP100 protein. Background Speckled protein 100 kDa (SP100), also known as SP100 nuclear antigen, was first identified as an antigen reactive with antibodies from patients with autoimmune disorders (1,2). The nuclear distribution of SP100 predominantly colocalizes with promyelocytic leukemia-nuclear bodies (PML-NBs) (3), which function as structures that regulate several diverse cellular functions, including gene regulation, DNA-damage responses, and apoptosis (4-6). This protein contains several isoforms, and the major isoform SP100-A has a calculated molecular weight of 54 kDa but exerts aberrant electrophoretic mobilities in SDS-polyacrylamide gel electrophoresis of approximately 100 kDa (1,2). SP100 has also been shown to undergo extensive post-translational modifications (PTM), including phosphorylation, ubiquitination, and SUMOylation. Moreover, PML-NBs play an important role in the PTM status of SP100, since they are thought to serve as hotspots for SUMOylation reactions, and PML depletion reduces SP100 SUMOylation (7-9). Alternate Names DKFZp686E07254; FLJ00340; FLJ34579; Lysp100b; nuclear antigen Sp100; Nuclear autoantigen Sp-100; Nuclear dot-associated Sp100 protein; SP100; SP100 nuclear antigen; SP100-HMG nuclear autoantigen; Speckled 100 kDa Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 80, 90, 130 Source/Isotype: Rabbit IgG