CST, 8013T, Rab17 (D2B2) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying RAB17. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Rab17 (D2B2) Rabbit Monoclonal Antibody (CST #8013) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Rab17 (D2B2) Rabbit Monoclonal Antibody detects endogeneous levels of total Rab17 protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe173 of human Rab17 protein. Background Rab17 belongs to the Rab family of small Ras-like GTPases. It is specifically expressed in epithelial cells and is upregulated during cell polarization (1). Immunofluorescence staining studies indicate that Rab17 is associated with the perinuclear recycling endosome in nonpolarized epithelial cells and with the apical recycling endosome in polarized epithelial cells (2). The function of Rab17 remains unclear. Reports of Rab17 colocalization with internalized IgA in the apical endosome suggest that it may regulate receptor-mediated transcytosis (3). Rab17 has also been shown to regulate melanocytic filopodia formation and melanosome trafficking. siRNA knockdown of Rab17 in melanoma cells induces melanosome accumulation in the cell periphery (4). Alternate Names FLJ12538; RAB17; RAB17, member RAS oncogene family; Ras-related protein Rab-17 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 23 Source/Isotype: Rabbit IgG