CST, 80182S, DGK zeta (F6W7N) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying DGKZ. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, DGK zeta (F6W7N) Rabbit Monoclonal Antibody (CST #80182) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity DGK zeta (F6W7N) Rabbit Monoclonal Antibody recognizes endogenous levels of total DGKζ protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human DGKζ protein. Background Diacylglycerol kinases (DGKs) are a unique family of enzymes that phosphorylate the signaling molecule diacylglycerol (DAG) and convert it to phosphatidic acid (PA) (1). DGKs affect various cellular processes by regulating DAG and PA concentrations, including immune signaling, glucose uptake, and neuronal remodeling (2). There are ten DGK isoforms in mammals (α, β, γ, δ, ε, ζ, η, θ, ι, and κ); these isoforms share a catalytic domain but differ in subcellular localization, tissue specificity, and overall function (3). DGK isoform zeta (DGKζ) is one of the primary isoforms expressed in T cells, where it negatively regulates TCR signaling (4). DGKζ attenuates TCR activation by blocking DAG-mediated signaling of downstream effector molecules, which can result in T cell anergy (5,6). Knockout or inhibition of DGKζ has been shown to reverse T cell anergy and enhance the cytotoxicity of chimeric antigen receptor T cells (CAR-Ts), suggesting that DGKζ can be targeted for immunotherapies (7,8). DGKζ may also play a role in cancer, where it is associated with cell proliferation and metastasis in cervical and triple-negative breast cancer (9,10). Alternate Names DAG kinase zeta; DAGK5; DAGK6; DGK-zeta; DGKZ; DGKZ iso1; Diacylglycerol kinase zeta; diacylglycerol kinase, zeta 104kDa; Diglyceride kinase zeta; hDGKzeta Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 110, 140 Source/Isotype: Rabbit IgG