CST, 80231P, STING (D2P2F) Rabbit Monoclonal Antibody (Biotinylated)

Monoclonal Antibody for studying STING. Validated for Western Blotting,Peptide ELISA (DELFIA). Highly specific and rigorously validated in-house, STING (D2P2F) Rabbit Monoclonal Antibody (Biotinylated) (CST #80231) is ready to ship. Product Usage Information Biotinylated antibodies are ideal for immunoassay technologies and high-throughput ELISA platforms that require antibody pairs where both antibodies are from the same host. Platforms utilizing biotinylated antibodies include, but are not limited to, MSD, xMAP, Quanterix Simoa, AlphaLISA, AlphaScreen, HTRF, LANCE, and TR-FRET. Optimal dilutions/working concentrations should be determined by the end user. Please contact us if you require the antibody clone biotinylated at a different concentration, a carrier-free formulation, or a more customized packaging solution. Storage Supplied in 140 mM NaCl, 3 mM KCl, 10 mM sodium phosphate (pH 7.4) dibasic, 2 mM potassium phosphate monobasic, 2 mg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity STING (D2P2F) Rabbit Monoclonal Antibody (Biotinylated) recognizes endogenous levels of total STING protein. Species Reactivity: Human, Mouse, Hamster Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu328 of human STING protein. Background Stimulator of interferon genes (STING, TMEM173, MITA) is a transmembrane adaptor protein that is a critical component of the cellular innate immune response to pathogenic cytoplasmic DNA (1,2). STING is a ubiquitously expressed protein found predominantly in the ER (1). The enzyme cGAMP synthase (cGAS) produces the second messenger cyclic-GMP-AMP (cGAMP) in response to cytoplasmic DNA (3,4). cGAMP binds and activates STING (3,4). In addition, detection of cytoplasmic DNA by nucleic acid sensors, including DDX41 or IFI16, results in STING activation (5,6). Following activation, STING translocates with TBK1 to perinuclear endosomes (7). The TBK1 kinase phosphorylates and activates interferon regulatory factors (IRFs) and NF-κB, which leads to the induction of type I interferon and other immune response genes (1,2,7). Alternate Names endoplasmic reticulum IFN stimulator; Endoplasmic reticulum interferon stimulator; ERIS; FLJ38577; hMITA; hSTING; Mediator of IRF3 activation; MITA; mitochondrial mediator of IRF3 activation; MPYS; N-terminal methionine-proline-tyrosine-serine plasma membrane tetraspanner; NET23; SAVI; Stimulator of interferon genes protein; stimulator of interferon protein; stimulator of interferon response cGAMP interactor 1; STING; STING-beta; STING1; TM173; TMEM173; Transmembrane protein 173 Specification REACTIVITY: H M Hm SENSITIVITY: Endogenous MW (kDa): 33, 35 Source/Isotype: Rabbit IgG