CST, 80331S, Tyrosine Hydroxylase (E2L6M) Rabbit Monoclonal Antibody (Alexa Fluor® 647 Conjugate)

Monoclonal Antibody for studying Tyrosine Hydroxylase. Validated for Immunohistochemistry (Paraffin),Immunofluorescence (Frozen),Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, Tyrosine Hydroxylase (E2L6M) Rabbit Monoclonal Antibody (Alexa Fluor^® 647 Conjugate) (CST #80331) is ready to ship. Product Usage Information Immunohistochemistry (Paraffin): 1:50 Immunofluorescence (Frozen): 1:50 - 1:100 Immunofluorescence (Immunocytochemistry): 1:100 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Immunohistochemistry (Paraffin), Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Tyrosine Hydroxylase (E2L6M) Rabbit Monoclonal Antibody (Alexa Fluor Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human tyrosine hydroxylase protein. Background Tyrosine hydroxylase (TH) catalyzes the rate-limiting step in the synthesis of the neurotransmitter dopamine and other catecholamines. TH functions as a tetramer, with each subunit composed of a regulatory and catalytic domain, and exists in several different isoforms (1,2). This enzyme is required for embryonic development since TH knockout mice die before or at birth (3). Levels of transcription, translation and post-translational modification regulate TH activity. The amino-terminal regulatory domain contains three serine residues: Ser9, Ser31, and Ser40. Phosphorylation at Ser40 by PKA positively regulates the catalytic activity of TH (4-6). Phosphorylation at Ser31 by CDK5 also increases the catalytic activity of TH through stabilization of TH protein levels (7-9). Alternate Names dystonia 14; DYT14; DYT5b; HTH-2; TH; TH2; TY3H; TYH; Tyrosine 3-hydroxylase; Tyrosine 3-monooxygenase; tyrosine hydroxylase Specification REACTIVITY: H M R SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG