CST, 80362T, GRK3 (D8G6V) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying GRK3. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, GRK3 (D8G6V) Rabbit Monoclonal Antibody (CST #80362) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity GRK3 (D8G6V) Rabbit Monoclonal Antibody recognizes endogenous levels of total GRK3 protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys454 of human GRK3 protein. Background G-protein-coupled receptor kinase 3 (GRK3), also known as beta-adrenergic receptor kinase 2 (beta-ARK2), is a member of the GRK family, which phosphorylates the activated form of G-protein-coupled receptors (GPCRs) and initiates the desensitization process of GPCR (1). GRK3 has been implicated in the phosphorylation of GPCRs, enabling their interaction with beta-arrestin, and facilitating their signaling through ERK1/2 phosphorylation (2). More recently, GRK3 was found to play a critical role in tumor progression through stimulation of angiogenesis; furthermore, GRK3 was found to be overexpressed in human prostate cancer, in particular in metastatic tumors (3). Alternate Names ADRBK2; adrenergic, beta, receptor kinase 2; ARBK2; BARK2; Beta-adrenergic receptor kinase 2; Beta-ARK-2; G protein-coupled receptor kinase 3; G-protein-coupled receptor kinase 3; GRK3 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 80 Source/Isotype: Rabbit IgG