CST, 80366S, CD16/CD32 (2.4G2) Rat Monoclonal Antibody (IF Formulated)

Monoclonal Antibody for studying FCGR2B iso2 mouse/FcGR2A mouse. Validated for Immunofluorescence (Frozen),Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, CD16/CD32 (2.4G2) Rat Monoclonal Antibody (IF Formulated) (CST #80366) is ready to ship. Product Usage Information Immunofluorescence (Frozen): 1:50 - 1:100 Immunofluorescence (Immunocytochemistry): 1:50 - 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. This product is stable for 60 months when stored at -20C. Protocol Available protocols: Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity CD16/CD32 (2.4G2) Rat Monoclonal Antibody (IF Formulated) recognizes endogenous levels of total CD16/CD32 protein. This antibody detects an epitope within the extracellular domain and is expected to detect all isoforms of CD16 and CD32. Staining of both microglia and astrocytes was seen when staining was done in 5XFAD mouse brain. Species Reactivity: Mouse Source / Purification This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. Background CD64 (FcgammaRI), CD32 (FcgammaRII), and CD16 (FcgammaRIII) are three classes of the immunoglobulin superfamily. CD64 has a high affinity for IgG with three Ig-like domains while CD32 and CD16 have low affinities with two Ig-like domains. Two genes encode CD16-A and CD16-B resulting only in a 6 amino acid difference in their ectodomains. However, CD16-A has a transmembrane anchor versus CD16-B, which has a glycosylphosphatidylinositol (1). CD64, CD32, and CD16 are membrane glycoproteins that are expressed by all immunologically active cells and trigger various immune functions (activate B cells, phagocytosis, antibody-dependent cellular cytotoxicity, immune complex clearance, and enhancement of antigen presentation) (2). CD16 cross-linking induces tyrosine phosphorylation (Tyr394) of Lck in NK cells (3). CD32 has tyrosine-based activation motifs in the cytoplasmic domain in contrast to CD16, which associates with molecules possessing these motifs (1). CD16 and CD32 are expressed on microglia within the brain (4). Alternate Names AI528646; CD16; CD32; F630109E10Rik; Fc gamma receptor IIB; Fc gamma receptor III; Fc gamma RIIB; Fc receptor, IgG, low affinity IIb; Fc receptor, IgG, low affinity III; Fc-gamma RII; Fc-gamma RIII; Fc-gamma-RII; Fc-gamma-RIIB; Fc[g]; Fc[g]RII; Fcg; Fcg receptor III; Fcgam; Fcgamm; FcgammaRIIB; FcgammaRIII; Fcgr; Fcgr2; Fcgr2a; FCGR2A; Fcgr2b; FCGR2B iso2; Fcgr3; FcgRII; Fcr-; Fcr-2; Fcr-3; FcRII; FcRIII; IgG Fc receptor II beta; IgG Fc receptor III; Low affinity immunoglobulin gamma Fc region receptor II; low affinity immunoglobulin gamma Fc region receptor II-b; Low affinity immunoglobulin gamma Fc region receptor III; Ly-1; Ly-17; Ly-m2; Ly-m20; LyM-; LyM-1; Lymphocyte antigen 17; OTTMUSP00000024374; OTTMUSP00000024375 Specification REACTIVITY: M SENSITIVITY: Endogenous Source/Isotype: Rat IgG2b