CST, 80409SF, Rb (D20) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying Rb mouse. Validated for Western Blotting,ELISA+. Highly specific and rigorously validated in-house, Rb (D20) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #80409) is ready to ship. Product Usage Information This product is the carrier free version of product #9313. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 9313 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Rb (D20) Rabbit Monoclonal Antibody (BSA and Azide Free) detects endogenous levels of total Rb protein. Species Reactivity: Human, Mouse, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ile419 of mouse Rb. Background The retinoblastoma tumor suppressor protein Rb regulates cell proliferation by controlling progression through the restriction point within the G1-phase of the cell cycle (1). Rb has three functionally distinct binding domains and interacts with critical regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase, and proteins with a conserved LXCXE motif (2-4). Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression (5). Rb inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4/6 followed by cyclin E-CDK2 phosphorylation (6). Specificity of different CDK/cyclin complexes has been observed (6-8) and cyclin D1 is required for Ser780 phosphorylation (9). Alternate Names p110-RB1; pp105; pRb; Rb; RB transcriptional corepressor 1; Rb-; Rb-1; Rb1; retinoblastoma 1; Retinoblastoma-associated protein Specification REACTIVITY: H M Mk SENSITIVITY: Endogenous MW (kDa): 110 Source/Isotype: Rabbit IgG