CST, 8049S, DcR2 (D13H4) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Trail-R4/DcR2. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, DcR2 (D13H4) Rabbit Monoclonal Antibody (CST #8049) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity DcR2 (D13H4) Rabbit Monoclonal Antibody recognizes endogenous levels of total DcR2 protein. This antibody may detect an unidentified protein of 30 kDa in some cell lines. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly270 of human DcR2 protein. Background The tumor necrosis factor receptor family, which includes TNF-RI, Fas, DR3, DR4, DR5, and DR6, plays an important role in the regulation of apoptosis in various physiological systems (1,2). The receptors are activated by a family of cytokines that include TNF, FasL, and TNF-related apoptosis-inducing ligand (TRAIL). They are characterized by a highly conserved extracellular region containing cysteine-rich repeats and a conserved intracellular region of about 80 amino acids termed the death domain (DD). The DD is important for transducing the death signal by recruiting other DD containing adaptor proteins (FADD, TRADD, RIP) to the death-inducing signaling complex (DISC), resulting in activation of caspases. Death receptor signaling can be controlled by a family of decoy receptors (DcR1, DcR2, and DcR3) that lack a cytoplasmic DD and inhibit death receptor-mediated apoptosis by competing for ligand binding (3-5). Expression of decoy receptors can contribute to chemosensitivity and may provide a mechanism for regulation of apoptosis in certain types of cancer (6-8). Alternate Names CD264; DCR2; Decoy receptor 2; decoy with truncated death domain; TNF receptor superfamily member 10d; TNF receptor-related receptor for TRAIL; TNF-related apoptosis-inducing ligand receptor 4; TNFRSF10D; TR10D; TRAIL receptor 4; TRAIL receptor with a truncated death domain; TRAIL-R4; TRAILR4; TRUNDD; Tumor necrosis factor receptor superfamily member 10D; tumor necrosis factor receptor superfamily, member 10d, decoy with truncated death domain Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 45-60 Source/Isotype: Rabbit IgG