CST, 80508S, HJURP (D3A8Z) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying FLEG1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, HJURP (D3A8Z) Rabbit Monoclonal Antibody (CST #80508) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity HJURP (D3A8Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total HJURP protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly672 of human HJURP protein. Background CENP-A is an essential histone H3 variant that replaces canonical histone H3 in centromeric heterochromatin. The inherited localization of the centromere is specified by CENP-A (1). CENP-A deposition to the correct chromosomal location in early G1 phase is regulated by the Mis18 complex, which recruits the CENP-A assembly factor/chaperone protein HJURP (Holliday Junction Recognition Protein) (2-3). Dimerization of HJURP is required for its activity (4), and phosphorylation by cyclin dependent kinases is required for temporal regulation of HJURP recruitment (5). Overexpression of HJURP causes chromosome loss in yeast and mitotic defects in mammalian cells (6). Further, downregulation of HJURP expression has been associated with replicative senescence in human cells (7). Research studies indicate that HJURP may have prognostic value in human breast cancer and high grade gliomas (8-10). Alternate Names 14-3-3-associated AKT substrate; DKFZp762E1312; FAKTS; fetal liver expressing gene 1; Fetal liver-expressing gene 1 protein; FLEG1; hFLEG1; HJURP; Holliday junction recognition protein; Up-regulated in lung cancer 9; URLC9 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 90 Source/Isotype: Rabbit IgG