CST, 8050S, Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit Monoclonal Antibody (Magnetic Bead Conjugate)

Monoclonal Antibody for studying . Validated for Immunoprecipitation. Highly specific and rigorously validated in-house, Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit Monoclonal Antibody (Magnetic Bead Conjugate) (CST #8050) is ready to ship. Product Usage Information Immunoprecipitation: 1:20 Storage Supplied in PBS Buffer (pH 7.2), 0.1% Tween ® 20. Store at 4°C. Do not aliquot the antibodies. Protocol Available protocols: Immunoprecipitation Specificity / Sensitivity Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit Monoclonal Antibody (Magnetic Bead Conjugate) recognizes peptides and proteins containing phospho-Ser/Thr preceded by Arg at the -3 position. There is some preference observed for peptides that contain phospho-Ser/Thr preceded by Arg at both positions -5 and -3. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.) Species Reactivity: All Species Expected Source / Purification Monoclonal antibody is produced by immunizing animals with synthetic phospho-Akt substrate peptides. Background An important class of kinases, referred to as Arg-directed kinases or AGC-family kinases, includes cAMP-dependent protein kinase (PKA), cGMP-dependent protein kinase (PKG), protein kinase C, Akt, and RSK. These kinases share a substrate specificity characterized by Arg at position -3 relative to the phosphorylated Ser or Thr (1,2). Akt plays a central role in mediating critical cellular responses including cell growth and survival, angiogenesis, and transcriptional regulation (3-5). While a number of Akt substrates are known (such as GSK-3, Bad, and caspase-9) many important substrates await discovery. Akt phosphorylates substrates only at Ser/Thr in a conserved motif characterized by Arg at positions -5 and -3 (6). Phospho-Akt substrate-specific antibodies from Cell Signaling Technology are powerful tools for investigating the regulation of phosphorylation by Akt and other Arg-directed kinases, as well as for high throughput kinase drug discovery. Specification REACTIVITY: All SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG