CST, 80517S, PD-L1 (F3Z1T) Rabbit Monoclonal Antibody (IHC Formulated)

Monoclonal Antibody for studying CD274 mouse. Validated for Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, PD-L1 (F3Z1T) Rabbit Monoclonal Antibody (IHC Formulated) (CST #80517) is ready to ship. Product Usage Information Immunohistochemistry (Paraffin): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 56005 . Protocol Available protocols: Immunohistochemistry (Paraffin) Specificity / Sensitivity PD-L1 (F3Z1T) Rabbit Monoclonal Antibody (IHC Formulated) recognizes endogenous levels of total mouse PD-L1 protein. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the extracellular domain of mouse PD-L1 protein. PD-L1 (F3Z1T) is the preferred clone for immunohistochemical analysis of mouse tissues. Background Programmed cell death 1 ligand 1 (PD-L1, B7-H1, CD274) is a member of the B7 family of cell surface ligands that regulate T cell activation and immune responses. The PD-L1 ligand binds the PD-1 transmembrane receptor and inhibits T cell activation. PD-L1 was discovered following a search for novel B7 protein homologs and was later shown to be expressed by antigen presenting cells, activated T cells, and tissues including placenta, heart, and lung (1-3). Similar in structure to related B7 family members, PD-L1 protein contains extracellular IgV and IgC domains and a short, cytoplasmic region. Research studies demonstrate that PD-L1 is expressed in several tumor types, including melanoma, ovary, colon, lung, breast, and renal cell carcinomas (4-6). Expression of PD-L1 in cancer is associated with tumor-infiltrating lymphocytes, which mediate PD-L1 expression through the release of interferon gamma (7). Additional research links PD-L1 expression to cancers associated with viral infections (8,9). Alternate Names A530045L16Rik; B7 homolog 1; B7-H1; B7h1; Cd274; CD274 antigen; OTTMUSP00000015826; PD-; PD-L1; PD1L1; PDCD1 ligand 1; Pdcd1l; Pdcd1l1; Pdcd1lg1; Pdl1; Programmed cell death 1 ligand 1; Programmed death ligand 1 Specification REACTIVITY: M SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG