CST, 8052T, TCF11/NRF1 (D5B10) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TCF11/NRF1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, TCF11/NRF1 (D5B10) Rabbit Monoclonal Antibody (CST #8052) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity TCF11/NRF1 (D5B10) Rabbit Monoclonal Antibody recognizes endogenous levels of total TCF11 protein. Species Reactivity: Human, Mouse, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly129 of human TCF11 protein. Background Transcription factor 11 (TCF11) is a basic leucine zipper transcription factor. It is also referred to as Nuclear factor E2-related factor 1 (NRF1). TCF11 was initially reported to activate erythroid-specific, human globin gene expression (1). It plays an essential role during embryonic development (2). It also associates with other transcription factors, such as Jun proteins, to transcriptionally control antioxidant response element (ARE)-mediated expression in response to antioxidants and xenobiotics (3-5). TCF11 has been shown to regulate proteasomal degradation and mediate the proteasome recovery pathway after proteasome inhibition (6,7). TCF11 is ubiquitously expressed (8) and several isoforms have been reported. The 120 kDa form exists in the endoplasmic reticulum (ER) membrane under normal conditions. Upon proteasome inhibition, TCF11 translocates to the nucleus (7). The 65 kDa N-terminal-truncated form is constitutively localized to the nucleus (9,10). TCF11 protein levels are regulated by ubiquitination and proteasomal-mediated degradation (11); proteasome inhibitors stabilize TCF11. Alternate Names Endoplasmic reticulum membrane sensor NFE2L1; FLJ00380; HBZ17; LCR-F1; Locus control region-factor 1; NF-E2-related factor 1; NF2L1; NFE2-related factor 1; NFE2L1; NRF1; nuclear factor (erythroid-derived 2)-like 1; Nuclear factor erythroid 2-related factor 1; nuclear factor, erythroid 2 like 1; Nuclear factor, erythroid derived 2, like 1; Protein NRF1, p110 form; Protein NRF1, p120 form; TCF-11; TCF11; Transcription factor 11; transcription factor 11 (basic leucine zipper type); Transcription factor HBZ17; Transcription factor LCR-F1; Transcription factor NRF1 Specification REACTIVITY: H M Mk SENSITIVITY: Endogenous MW (kDa): 120-140 Source/Isotype: Rabbit IgG