CST, 80561T, Parvalbumin (E8N2U) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Parvalbumin mouse. Validated for WB,IP,IHC,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, Parvalbumin (E8N2U) Rabbit Monoclonal Antibody (CST #80561) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:50 - 1:200 Immunofluorescence (Frozen): 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody . For a carrier free (BSA and azide free) version of this product see product # 51358 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen) Specificity / Sensitivity Parvalbumin (E8N2U) Rabbit Monoclonal Antibody recognizes endogenous levels of total parvalbumin protein. In mouse models, this antibody nonspecifically labels muscle in small intestine and juxtaglomerular cells in kidney by immunofluorescence. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human parvalbumin protein. Background Parvalbumin (PV, PVALB) is a member of the EF-hand family of calcium-binding proteins, containing EF-hand domains with a high affinity for calcium and magnesium (1). Parvalbumin is expressed in both the soma and in processes of specific subclasses of GABAergic interneurons. Parvalbumin is a slow calcium buffer, meaning it accelerates the initial phase of decay of calcium after an action potential, which likely inhibits facilitation and is thought to regulate short-term synaptic plasticity (2,3). Altered function of PV positive interneurons has been implicated in many neurological diseases, such as Alzheimer's disease, autism spectrum disorder, schizophrenia, and bipolar disorder (4). Alternate Names Parv; parvalbumin; Parvalbumin alpha; PRVA; PV; Pva; Pvalb Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 12 Source/Isotype: Rabbit IgG