CST, 8066S, NRBF-2 Antibody

Polyclonal Antibody for studying NRBF2. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, NRBF-2 Antibody (CST #8066) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity NRBF-2 Antibody recognizes endogenous levels of total NRBF-2 protein. This antibody may cross-react with NRBF-1/COPR-1. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu145 of human NRBF-2 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Nuclear Receptor Binding Factor-2 (NRBF-2), also referred to as Comodulator of PPAR and RXRα-2 (COPR-2), has been shown to interact with the AF-2 region of several nuclear hormone receptors with varying affinities such as PPARα, RARα, RARγ, and RXRα (1,2). NRBF-2 contains a LLYLL motif, which matches the LXXLL NR box consensus and is required for functional NRBF-2/nuclear receptor complex formation and repression of receptor function. NRBF-2 also contains a unique autonomous activation domain and, thus, does not completely abrogate nuclear receptor function, suggesting that NRBF-2 might serve as a molecular rheostat to fine-tune the transcriptional activity of liganded nuclear receptors (1,2). Alternate Names Comodulator of PPAR and RXR; comodulator of PPAR and RXR 1; comodulator of PPAR and RXR 2; COPR; COPR1; COPR2; DKFZp564C1664; FLJ30395; NRBF-2; NRBF2; nuclear receptor binding factor 2; nuclear receptor binding factor-2; Nuclear receptor-binding factor 2 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 40 SOURCE: Rabbit