CST, 8068T, Cleaved GGA3 (Asp313) (D16H3) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying GGA3 (Asp313) cleaved. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Cleaved GGA3 (Asp313) (D16H3) Rabbit Monoclonal Antibody (CST #8068) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Cleaved GGA3 (Asp313) (D16H3) Rabbit Monoclonal Antibody recognizes endogenous levels of GGA3 protein only when cleaved at Asp313. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp313 of human GGA3 protein. Background GGA3 is a member of the GGA family of proteins which also includes GGA1 and GGA2. These proteins consist of four distinct segments: a VHS domain that binds the di-leucine sorting signal DXXLL; a GAT domain that binds Arf-GTP; a hinge region that recruits clathrin; and a GAE domain that has sequence similarity to γ-adaptin and recruits a number of proteins. Arf1-GTPase recruits GGA3 to the trans-Golgi network. GGAs sort acid hydrolases to the lysosome and are involved in transporting proteins containing the DXXLL signal from the Golgi complex to the endosome (1). During apoptosis or cerebral ischemia, GGA3 is cleaved by caspase-3 at Asp313, reducing GGA3 levels and lysosomal degradation of β-secretase (BACE). The resulting elevated amount and activity of BACE plays a role in amyloid-β (Aβ) production, consistent with BACE elevation and Aβ accumulation in Alzheimer's Disease (2). Alternate Names ADP-ribosylation factor-binding protein GGA3; GGA3; golgi associated, gamma adaptin ear containing, ARF binding protein 3; golgi-associated, gamma adaptin ear containing, ARF binding protein 3; Golgi-localized, gamma ear-containing, ARF-binding protein 3; KIAA0154 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 32 Source/Isotype: Rabbit IgG