CST, 8076S, TFAM (D5C8) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TFAM. Validated for WB,WB,IP,IHC,IF,eCLIP. Available in 2 sizes. Highly specific and rigorously validated in-house, TFAM (D5C8) Rabbit Monoclonal Antibody (CST #8076) is ready to ship. Product Usage Information For more information about the RBP-eCLIP service please visit Eclipsebio . Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:100 - 1:400 Immunofluorescence (Immunocytochemistry): 1:100 - 1:200 eCLIP: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity TFAM (D5C8) Rabbit Monoclonal Antibody recognizes endogenous levels of total TFAM protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val225 of human TFAM protein. Background TFAM (Transcription Factor A, Mitochondrial; aka TCF6) is a member of the high-mobility group (HMG) proteins because it contains two HMG boxes. TFAM is a transcription factor for mitochondrial DNA (mtDNA), and enhances mtDNA transcription in a promoter-specific fashion in the presence of mitochondrial RNA polymerase and transcription factor B (1). Because the majority of ATP production depends on the mitochondrial respiratory chain, maintenance of the mitochondrial genome is critical for normal health. TFAM plays an essential role in the maintenance of mtDNA and thus, ATP production (2). TFAM binds to mtDNA both nonspecifically and in a sequence-specific manner. It is known to have a dual effect on mtDNA: protection of mtDNA and initiation of transcription from mtDNA (3). TFAM attenuates age-dependent impairment of the brain by preventing oxidative stress and mitochondrial dysfunctions in microglia (4). Alternate Names Mitochondrial transcription factor 1; mitochondrial transcription factor A; MTDPS15; MtTF1; mtTFA; TCF-6; TCF6; TCF6L1; TCF6L2; TCF6L3; TFAM; Transcription factor 6; transcription factor 6-like 1; Transcription factor 6-like 2; Transcription factor 6-like 2 (mitochondrial transcription factor); transcription factor 6-like 3; Transcription factor A, mitochondrial Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 24 Source/Isotype: Rabbit IgG