CST, 80775T, BACH2 (D3T3G) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying BACH2. Validated for Western Blotting,Immunoprecipitation,Chromatin IP. Available in 2 sizes. Highly specific and rigorously validated in-house, BACH2 (D3T3G) Rabbit Monoclonal Antibody (CST #80775) is ready to ship. Product Usage Information For optimal ChIP results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits Western Blotting: 1:1000 Immunoprecipitation: 1:200 Chromatin IP: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Chromatin IP Specificity / Sensitivity BACH2 (D3T3G) Rabbit Monoclonal Antibody recognizes endogenous levels of total BACH2 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala304 of human BACH2 protein. Background The transcription regulator BTB and CNC homolog 2 (BACH2) is a bZIP domain-containing transcriptional repressor that dimerizes with MafK and binds Maf recognition elements (MAREs) to regulate transcription (1,2). BACH2 is part of a network of transcription factors that controls the transition of activated B cells into either antibody-producing plasma cells or memory B cells (3-5). Plasma cell differentiation requires the transcription factor Blimp1 (6). BACH2 suppresses expression of Blimp1 in activated B cells, which delays plasma cell differentiation and allows time for class switch recombination and somatic hypermutation (3-5). Genome-wide association studies have linked the genetic locus containing BACH2 to several immune-related disorders, including type 1 diabetes, celiac disease, Crohn's disease, and the skin condition known as vitiligo (7-10). Alternate Names BACH2; BTB and CNC homolog 2; BTB and CNC homology 1, basic leucine zipper transcription factor 2; BTB domain and CNC homolog 2; BTBD25; IMD60; Transcription regulator protein BACH2 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 130 Source/Isotype: Rabbit IgG