CST, 8080S, TFIIF-alpha Antibody

Polyclonal Antibody for studying TFIIF-alpha. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, TFIIF-alpha Antibody (CST #8080) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity TFIIF-alpha Antibody recognizes endogenous levels of total TFIIF-α protein. Species Reactivity: Human, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human TFIIF-α protein. Antibodies are purified by protein A and peptide affinity chromatography. Background TFIIF is a member of the group of general transcription factors that facilitate the binding of RNA polymerase II (Pol II) to promoter sequences as part of the pre-initiation complex (PIC) (1). TFIIF consists of subunits TFIIF-α (RAP74) and TFIIF-β (RAP30). It is involved in the stabilization of Pol II association with the PIC and selection of the transcription start site during transcription initiation (1,2). In addition to its role in transcription initiation, TFIIF has been shown to stimulate the transcription elongation activity of Pol II as well as dephosphorylation and recycling of Pol II during transcription termination (3-5). Alternate Names BTF4; General transcription factor IIF 74 kDa subunit; General transcription factor IIF subunit 1; general transcription factor IIF, polypeptide 1, 74kDa; GTF2F1; RAP74; T2FA; TF2F1; TFIIF; TFIIF-alpha; Transcription initiation factor IIF subunit alpha; Transcription initiation factor RAP74 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 74 SOURCE: Rabbit