CST, 80827S, ZNF281 (F5Z7H) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ZNF281. Validated for Chromatin IP. Available in 2 sizes. Highly specific and rigorously validated in-house, ZNF281 (F5Z7H) Rabbit Monoclonal Antibody (CST #80827) is ready to ship. Product Usage Information For optimal ChIP results, use 10 μL of antibody and 10 μg of chromatin (approximately 4 × 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Chromatin IP: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Chromatin IP Specificity / Sensitivity ZNF281 (F5Z7H) Rabbit Monoclonal Antibody recognizes endogenous levels of total ZNF281 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His756 of human ZNF281 protein. Background Zinc finger protein 281 (ZNF281), also known as ZFP281, ZBP-99, and ZNP-99, is a zinc finger-containing transcriptional factor. The protein binds to GC-rich sequences located in the regulatory regions of many genes to promote or repress target gene transcription (1). ZNF281 is involved in regulating many important biological processes. It promotes epithelial-mesenchymal transition by transcriptional upregulation of Snail (2), represses embryonic stem cell (ESC) pluripotency by down-regulation of Nanog transcription, an essential gene for cellular stemness (3,4), and orchestrates interconversion of pluripotent states of ESC by engaging expression of multiple genes such as and , and (5,6). DNA stability can be affected by ZNF281 in various ways. The protein facilitates DNA damage responses by controlling the expression of XRCC2 and XRCC4 (7), interacts with BRCA2 to prevent R-loop accumulation during DNA replication (8), and binds DNA breaks to facilitate DNA repair by non-homologous end joining (9). ZNF281 is upregulated and promotes tumor progression in most cancer types, such as colon cancer, pancreatic cancer, and breast cancer (10). ZFP281 locks early disseminated breast cancer cells in a dormant state and prevents cancer cell outgrowth in the lung metastatic niche (11). Alternate Names FLJ12859; FLJ14378; GC-box-binding zinc finger protein 1; GZP1; Transcription factor ZBP-99; ZBP-99; ZBP99; Zinc finger DNA-binding protein 99; Zinc finger protein 281; ZN281; ZNF281; ZNP-99; ZNP-99 transcription factor Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG