CST, 80916S, Stat1 (D1K9Y) Rabbit Monoclonal Antibody (PE Conjugate)

Monoclonal Antibody for studying Stat1. Validated for Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, Stat1 (D1K9Y) Rabbit Monoclonal Antibody (PE Conjugate) (CST #80916) is ready to ship. Product Usage Information Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Stat1 (D1K9Y) Rabbit Monoclonal Antibody (PE Conjugate) recognizes endogenous levels of total Stat1 protein. This antibody conjugate also cross-reacts with an unidentified protein of 150 kDa. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro688 of human Stat1 protein. Background The Stat1 transcription factor is activated in response to a large number of ligands (1) and is essential for responsiveness to IFN-α and IFN-γ (2,3). Phosphorylation of Stat1 at Tyr701 induces Stat1 dimerization, nuclear translocation, and DNA binding (4). Stat1 protein exists as a pair of isoforms, Stat1α (91 kDa) and the splice variant Stat1β (84 kDa). In most cells, both isoforms are activated by IFN-α, but only Stat1α is activated by IFN-γ. The inappropriate activation of Stat1 occurs in many tumors (5). In addition to tyrosine phosphorylation, Stat1 is also phosphorylated at Ser727 through a p38 mitogen-activated protein kinase (MAPK)-dependent pathway in response to IFN-α and other cellular stresses (6). Serine phosphorylation may be required for the maximal induction of Stat1-mediated gene activation. Alternate Names CANDF7; DKFZp686B04100; IMD31A; IMD31B; IMD31C; ISGF-3; signal transducer and activator of transcription 1; Signal transducer and activator of transcription 1-alpha/beta; signal transducer and activator of transcription 1, 91kD; signal transducer and activator of transcription 1, 91kDa; signal transducer and activator of transcription-1; STAT1; STAT91; Transcription factor ISGF-3 components p91/p84 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG