CST, 80920S, PNUTS (D8H5J) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PNUTS. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, PNUTS (D8H5J) Rabbit Monoclonal Antibody (CST #80920) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity PNUTS (D8H5J) Rabbit Monoclonal Antibody recognizes endogenous levels of total PNUTS protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys244 of human PNUTS protein. Background Protein phosphatase-1 nuclear targeting subunit (PNUTS) is one of the key regulators of protein phosphatase 1 (PP1) in the nucleus (1). Via interaction with PP1, PNUTS plays an essential role in multiple cellular processes, including chromatin decondensation (2), DNA damage response (3), and cardiomyocyte apoptosis (4). Notably, PNUTS also regulates the activity of two key tumor suppressors, Rb and p53, through inhibition of PP1 mediated dephosphorylation (5-7). Research studies indicate that PNUTS also sequesters PTEN in the nucleus through direct interaction and inhibits its tumor suppressor function (8). PNUTS is ubiquitously expressed and elevated PNUTS expression is observed in various cancers such as esophageal carcinoma, squamous cell carcinoma, and prostate cancer (1,8). Alternate Names CAT53; FB19; HLA-C associated transcript 53; MHC class I region proline-rich protein CAT53; p99; Phosphatase 1 nuclear targeting subunit; phosphatase nuclear targeting subunit; PNUTS; PP1-binding protein of 114 kDa; PP1R10; PP1RA; PPP1R10; Protein FB19; protein phosphatase 1 regulatory subunit 10; protein phosphatase 1, regulatory (inhibitor) subunit 10; protein phosphatase 1, regulatory subunit 10; R111; Serine/threonine-protein phosphatase 1 regulatory subunit 10; testis tissue sperm-binding protein Li 67n Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 115 Source/Isotype: Rabbit IgG