CST, 80941SF, Phospho-Tyrosine (9E9) Mouse Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying . Validated for ELISA+. Highly specific and rigorously validated in-house, Phospho-Tyrosine (9E9) Mouse Monoclonal Antibody (BSA and Azide Free) (CST #80941) is ready to ship. Product Usage Information This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Phospho-Tyrosine (9E9) Mouse Monoclonal Antibody (BSA and Azide Free) recognizes a broad range of tyrosine-phosphorylated proteins. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with phospho-tyrosine containing peptides. Background Tyrosine phosphorylation plays a key role in cellular signaling (1). Research studies have shown that in cancer, unregulated tyrosine kinase activity can drive malignancy and tumor formation by generating inappropriate proliferation and survival signals (2). Antibodies specific for phospho-tyrosine (3,4) have been invaluable reagents in these studies. The phospho-tyrosine monoclonal antibodies developed by Cell Signaling Technology are exceptionally sensitive tools for studying tyrosine phosphorylation and monitoring tyrosine kinase activity in high throughput drug discovery. Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Mouse IgG2a kappa